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25mM MgCl2
[2] Caruthers
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4 (2015): 4522
a 5’-dimethoxytrityl (DMT) group for 5’-hydroxyl protection
RNase R RNA (2'-O-TBDMS) Phosphoramidites 25mM MgCl2Product Information Ribonuclease R (RNase R) from E. coli, is a magnesium dependent 3' to 5' exoribonuclease that digests essentially all linear RNAs but does not digest lariat or circular RNA structures, or double stranded RNA with 3' overhangs shorter than 7 nucleotides. RNase R digests RNA from the 3' to 5' direction, releasing 5' monophosphate nucleotides to produce dinucleotide and trinucleotide end products. Resource: Recombinant E. coli
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