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30(1):448-467
Mix contains red tracer dye
Oligo dT magnetic beads
Dilution Buffer 1
2 µm filtered concentrated solution in 20 mM Tris
RNase HII, Glycerol-free(2 U/μL) _ 14541ES Size:1 KU 30(1):448-467RNase HII is a ribonuclease internal enzyme obtained from the Pyrococcus abyssi and recombinantly expressed in E. Coli. It recognizes the DNA rN DNA DNA double strand, cutting at the site where ribonucleotides are incorporated into DNA. However, its activity on single stranded RNA is very low, and it shows no cutting activity on dsDNA or ssDNA. RNase HII cuts at the site of a single ribonucleotide residue from the 5' end, producing a 5' phosphate
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