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Hieff Clone™ Zero TOPO-TA/Blunt Cloning Kit _ 10906ES Size:20 T Using different genes from mouse

SKU: 85405131654

4.1
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Description

Using different genes from mouse

Seasonal expressions of GPR41 and GPR43 in the colon of the wild ground squirrels (Spermophilus dauricus)

Biotinylated Human MICB Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus

The recommended working concentration of 6×His Tag peptides for elution of 6×His-Tag fusion proteins is 100-400 μg/mL

DNA Polymerase Activity Release Detection :In the HieffUNICONTM HotStart Taq Buffer (Mg2+  Plus) ,the activity release was higher than 95% when heated at 95℃ for 30 sec

Hieff Clone™ Zero TOPO-TA/Blunt Cloning Kit _ 10906ES Size:20 T Using different genes from mouseHieff Clone Zero TOPO TA Blunt Cloning Kit is developed based on the principle of a next generation topoisomerase that enables rapid and efficient ligation of DNA fragments. Coupled with a highly efficient blunt ending factor, this kit supports fast cloning of both blunt ended and sticky ended PCR products. Features Fast: Ligation completes in just 5 minutes. Highly Efficient: Virtually no self ligation; near 100% positive clone rateno blue white

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